scirpy.tl.repertoire_overlap

scirpy.tl.repertoire_overlap(adata, groupby, *, target_col='clone_id', overlap_measure='jaccard', overlap_threshold=None, fraction=False, inplace=True, added_key='repertoire_overlap', airr_mod='airr')

Compute distance between cell groups based on clonotype overlap.

Adds parwise overlaps, distance matrix and linkage to uns.

Warning

This function is experimental and is likely to change in the future.

Parameters:

• adata (Union[AnnData, MuData, DataHandler]) – AnnData or MuData object that contains AIRR information.

• groupby (str) – Column with group labels (e.g. samples, tussue source, diagnosis, etc).

• target_col (str (default: 'clone_id')) – Category that overlaps among groups (clone_id by default, but can in principle be any group or cluster)

• overlap_measure (str (default: 'jaccard')) – Any distance measure accepted by scipy.spatial.distance; by default it is jaccard.

• overlap_threshold (Optional[float] (default: None)) – The minimum required weight to accept presence.

• fraction (Union[str, bool] (default: False)) – If True, compute fractions of abundances relative to the groupby column rather than reporting abosolute numbers. Alternatively, a column name can be provided according to that the values will be normalized or an iterable providing cell weights directly. Setting it to False, assigns equal weight to all cells.

• inplace (bool (default: True)) – Whether results should be added to uns or returned directly.

• added_key (str (default: 'repertoire_overlap')) – Results will be added to uns under this key.

• airr_mod (str (default: 'airr')) – Name of the modality with AIRR information is stored in the MuData object. if an AnnData object is passed to the function, this parameter is ignored.

Return type:

Optional[tuple[DataFrame, ndarray, ndarray]]

Returns:

A DataFrame used by the pairwise scatterplot, distance matrix and linkage.